Molecular characterization and functional analysis of Eimeria tenella citrate synthase

Chicken coccidiosis, attributable to an obligate intracellular protozoan parasite of the genus Eimeria, is a serious parasitic illness within the intensively reared poultry trade. Due to the widespread use of anticoccidial medication, resistance has grow to be an inevitable drawback. In our earlier research, Eimeria tenella citrate synthase (EtCS) was discovered to be up-expressed in two drug-resistant strains (diclazuril-resistant and maduramycin-resistant strains) in comparison with drug-sensitive pressure by RNA sequence. In this research, we cloned and expressed EtCS and receive its polyclonal antibodies.

Quantitative real-time polymerase chain (qPCR) reactions and Western blots have been used to investigate the transcription and translation ranges of EtCS in delicate and three drug-resistant strains. Compared with the delicate pressure, the transcription of EtCS was each considerably upregulated in diclazuril-resistant and maduramycin-resistant strains, however was not considerably totally different in salinomycin-resistant pressure. No vital distinction was seen in translation degree within the three drug-resistant strains. Indirect immunofluorescence indicated that EtCS was primarily situated within the cytoplasm of sporozoites aside from posterior refractile our bodies and within the cytoplasm and floor of merozoites.

Anti-rEtCS antibody has inhibitory results on E. tenella sporozoite invasion of DF-1 cells and the inhibition charge is greater than 83%. Binding of the protein to rooster macrophage (HD11) cells was confirmed by immunofluorescence assays. When macrophages have been handled with rEtCS, secretion of nitric oxide and cell proliferation of the macrophages have been considerably decreased. These outcomes confirmed that EtCS could also be associated to host cell invasion of E. tenella and contain within the improvement of E.tenella resistance to some medication.

Establishment of main rooster embryo myoblast cell tradition, antigenic epitopes prediction and manufacturing of anti activin receptor kind IIB polyclonal antibody in rooster

The detection of activin receptor typeIIB (ACTRIIB) protein, a distinguished damaging muscle progress regulator has paramount worth in augmenting progress traits by molecular breeding schemes in rooster. The research was formulated to ascertain main rooster embryo myoblast tradition (CEM) utilizing ninth and 18th day chick embryos and to develop antibodies for immunodetection of ACTRIIB protein. The physicochemical and structural attributes of the ACTRIIB sequence have been evaluated to establish substantial antigenic areas. The ACTRIIB sequence was transfected into CEM and expressed protein was injected subcutaneously into rats to provide hyperimmune serum.

The common propensity of protein sequence for beta turns, floor accessibility, chain flexibility, antigenicity, hydrophilicity and linear epitopes was 0.978, 1.000, 0.991, 1.038, 1.258 and 0.512, respectively. The ninth day CEM exhibited confluency (80-90%) sooner than the 18th day. The expression of myogenic regulatory components in ninth day myoblasts was larger than the 18th day by 7.28, 5.16, 6.28 and 6.93 folds for MYF5, MRF4, MYOG and MYOD, respectively. The ACTRIIB mRNA was downregulated by 2.54 folds on the ninth day in comparison with the 18th day myoblasts and protein assorted considerably between ninth and 18th day myoblasts. The CEM tradition might be harnessed unequivocally to analyze molecular mechanisms underlying muscle progress in addition to elevating antibodies.

Campylobacteriosis is a illness in people attributable to the an infection from Campylobacter spp. Human circumstances are primarily on account of Campylobacter jejuni, though C. coli may cause gastroenteritis in people as properly. The micro organism are commensal in rooster tract and might be contaminated into rooster merchandise throughout processing. Obviously, detecting reagents akin to a particular antibody is important for the event of immune-based detection strategies for C. jejuni or C. coli. In this research, in silico methods have been used to design a chimeric recombinant antigen, named multiepitope antigen (MEA), for the manufacturing of particular polyclonal antibody.

To design MEA polypeptide primarily based on C. jejuni fibronectin-binding protein or CadF, 4 conserved and distinctive antigenic peptides have been recognized and fused collectively instantly. The C. jejuni CadF-based MEA polypeptide fused with two single six-histidine tags at each C- and N-terminal ends was expressed underneath Escherichia coli expression system. The recombinant MEA was efficiently produced and purified by Ni-NTA resin with a excessive passable yield. Indirect ELISA outcomes confirmed that anti-MEA polyclonal antibody derived from rabbit serum had a titer of 16,000, indicating excessive antigenicity of MEA polypeptide. Dot blot outcomes additionally confirmed that the produced anti-MEA antibody might particularly acknowledge each C. jejuni and C. coli entire cells as anticipated whereas there was no cross-reactivity to non-Campylobacter spp. examined on this research.

Molecular characterization and functional analysis of Eimeria tenella citrate synthase

Cloning and prokaryotic expression of the rooster liver kinase B1 (LKB1) and its localization in liver, coronary heart and hypothalamus

Liver kinase B1 (LKB1) is a member of the serine/threonine kinase household, which performs an indispensable function within the organism of animals. In the present research, the rooster LKB1 protein gene was amplified by PCR primarily based on the primers and cDNA templates. Then, the cloning vector was constructed and the goal gene was cloned. After that, the goal gene was inserted into the expression vector to assemble the recombinant plasmid. The recombinant plasmid was remodeled into BL21 (DE3) host cells and the LKB1 recombinant proteins have been efficiently expressed by utilizing Isopropyl-β-D-thiogalactopyranoside (IPTG). Finally, purified LKB1 proteins have been used as antigen and the rabbit-derived antiserums have been collected. The antiserum titer decided by ELISA was not lower than 1:128000.

Genomic DNA from Mouse Normal Tissue: Lung
D1334152	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Lung
D1434152	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

96 Well Lung Tumor Genomic DNA Plate
D8235152-1	Biochain	1 plate	EUR 316

FFPE Genomic DNA - Human Adult Normal Tissue: Lung
D2234152	Biochain	2 ug	EUR 599
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Genomic DNA
BIO-35025	Bioline	500µl @ 200ng/µl	Ask for price

Human Genomic DNA
X11000	EpiGentek	0.2 ml	Ask for price
Description: reagents widely cited

Genomic DNA - Human Diabetic Tissue: Lung, from a single donor
D1236152Dia	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Asthma: Lung, from a single donor
D1236152Ld-1	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Bronchitis: Lung, from a single donor
D1236152Ld-2	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Emphysema: Lung, from a single donor
D1236152Ld-3	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Pneumonia: Lung, from a single donor
D1236152Ld-4	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Lung, from a single donor
D1236152Lup	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Male
D1234999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Female
D1234999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Brain Genomic DNA
X11001	EpiGentek	10 µg	Ask for price
Description: kits suitable for this type of research

Genomic DNA Kit
20-abx098076	Abbexa	EUR 439.00 EUR 244.00	200 rxns 50 rxns
Shipped within 5-10 working days.

FFPE and Frozen Matching Pair Genomic DNA: Human Tumor Tissue: Lung
D8235152-FP	Biochain	2 x 2 ug	EUR 1957
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Liver Cirrhosis: Lung, from a single donor
D1236152Lcs	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Pulmonary embolism: Lung, from a single donor
D1236152Ld-5	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Human Adult Normal Tissue: Lung, from a single donor
D1234152	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Human Tumor Tissue: Lung Tumor, from a single donor
D1235152	Biochain	50 ug	EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA Isolation Kit
55R-1362	Fitzgerald	50 assays	EUR 385
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA - Mouse Male
D1334999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Mouse Female
D1334999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Male
D1434999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Female
D1434999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Male
D1734999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Female
D1734999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Male
D1834999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Female
D1834999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Male
D1934999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Female
D1934999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Male
D1B34999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Female
D1B34999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Male
D1C34999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Female
D1C34999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Male
D1H34999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Female
D1H34999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Male
D1O34999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Female
D1O34999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Genomic DNA Kit
20-abx098077	Abbexa	EUR 411.00 EUR 230.00	200 rxns 50 rxns
Shipped within 5-10 working days.

Bacteria Genomic DNA Kit
20-abx098080	Abbexa	EUR 105.00 EUR 244.00	2 rxns 50 rxns
Shipped within 5-10 working days.

Micro Genomic DNA Kit
20-abx098242	Abbexa	EUR 105.00 EUR 328.00	2 rxns 50 rxns
Shipped within 5-10 working days.

Blood Genomic DNA Kit
abx098868-50rxns	Abbexa	50 rxns	EUR 272
Shipped within 5-10 working days.

Plant Genomic DNA Kit
abx294004-50preps	Abbexa	50 preps	EUR 398
Shipped within 5-12 working days.

Animal Genomic DNA Kit
abx294005-100preps	Abbexa	100 preps	EUR 523
Shipped within 5-12 working days.

Animal Genomic DNA Kit
abx294005-50preps	Abbexa	50 preps	EUR 398
Shipped within 5-12 working days.

Genomic DNA Extraction Kit
K5016005	Biochain	1 kit	EUR 329

Genomic DNA Isolation Kit
K281-50	Biovision		EUR 272

Genomic DNA Isolation Kit
K2118-50	ApexBio	50 assays	EUR 321

Soil Genomic DNA Kit
K1411-250	Biovision		EUR 561

Soil Genomic DNA Kit
K1411-50	Biovision		EUR 316

Insect Genomic DNA Kit
K1412-250	Biovision		EUR 561

Insect Genomic DNA Kit
K1412-50	Biovision		EUR 316

Mollusc Genomic DNA Kit
K1413-250	Biovision		EUR 561

Mollusc Genomic DNA Kit
K1413-50	Biovision		EUR 316

Yeast Genomic DNA Kit
K1414-250	Biovision		EUR 561

Yeast Genomic DNA Kit
K1414-50	Biovision		EUR 316

Fungal Genomic DNA Kit
K1415-250	Biovision		EUR 561

Fungal Genomic DNA Kit
K1415-50	Biovision		EUR 316

Fully CpG Methylated Human Genomic DNA
X10000	EpiGentek	10 µg/100 µl	Ask for price
Description: The best epigenetics products

Genomic DNA - Cynomolgus Monkey Male
D1534999-Cy-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Cynomolgus Monkey Female
D1534999-Cy-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Male
D1534999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Female
D1534999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Male
D1G34999-G01	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Female
D1G34999-G02	Biochain	100 ug	EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Genomic DNA extraction buffer
20-abx098075	Abbexa	EUR 286.00 EUR 105.00	100 ml 1.2 ml
Shipped within 5-10 working days.

Marine Animal Genomic DNA Kit
20-abx098079	Abbexa	EUR 105.00 EUR 244.00	2 rxns 50 rxns
Shipped within 5-10 working days.

FFPE Tissue Genomic DNA Kit
abx098866-50rxns	Abbexa	50 rxns	EUR 272
Shipped within 5-10 working days.

ISOLATE II Genomic DNA Kit
BIO-52065	Bioline	10 preps	Ask for price

ISOLATE II Genomic DNA Kit
BIO-52066	Bioline	50 preps	Ask for price

ISOLATE II Genomic DNA Kit
BIO-52067	Bioline	250 preps	Ask for price

Genomic DNA Extraction KitAnimal Tissue
FYG102-100P	Yeastern Biotech	100 Preps	Ask for price

Genomic DNA Extraction KitAnimal Tissue
FYG102-300P	Yeastern Biotech	300 Preps	Ask for price

Genomic DNA Extraction KitAnimal Tissue
FYG102-50P	Yeastern Biotech	50 Preps	Ask for price

Genomic DNA Extraction Maxi KitPlant
FYG104-10P	Yeastern Biotech	10 Preps	Ask for price

Genomic DNA Extraction Maxi KitPlant
FYG104-25P	Yeastern Biotech	25 Preps	Ask for price

AccuRT Genomic DNA Removal Kit
G488	ABM	200 Reactions	EUR 82

Genomic DNA Extraction and Purification
EP10012	Neuromics	100 Tests	EUR 505

Bacterial Genomic DNA Isolation Kit
K309-100	Biovision		EUR 376

Zenoquick Genomic DNA Purification Kit
Z2003-200	GenDepot	200 preps	EUR 631

Zenoprep Genomic DNA Purification Kit
Z2004-400	GenDepot	400 preps	EUR 550

FFPE Genomic DNA - Human Tumor Tissue: Breast
D2235086	Biochain	2 ug	EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Colon
D2235090	Biochain	2 ug	EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Pancreas
D2235188	Biochain	2 ug	EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Stomach
D2235248	Biochain	2 ug	EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Tumor Tissue: Uterus
D2235274	Biochain	2 ug	EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP	CORNING	1/pk	EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB	CORNING	1/pk	EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Genomic DNA Kit (No RNase A)
20-abx098862	Abbexa	EUR 398.00 EUR 230.00	200 rxns 50 rxns
Shipped within 5-10 working days.

Genomic DNA Extraction Mini KitPlant Tissue
FYG103-100P	Yeastern Biotech	100 Preps	Ask for price

Easy3-Plant Genomic DNA Isolation Kit
FYG503-100P	Yeastern Biotech	100 Preps	Ask for price

Easy3-Plant Genomic DNA Isolation Kit
FYG503-500P	Yeastern Biotech	500 Preps	Ask for price

Mammalian Cell Genomic DNA Isolation Kit
K967-100	Biovision		EUR 381

Plant Tissue Genomic DNA Isolation Kit
K316-100	Biovision		EUR 376

Rapid Animal Genomic DNA Extraction Kit
AT4780	Bio Basic	10Preps	EUR 69.03
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Animal Genomic DNA Extraction Kit
AT4781	Bio Basic	50Preps	EUR 126.13
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Animal Genomic DNA Extraction Kit
AT4782	Bio Basic	250preps	EUR 354.5
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Bacteria Genomic DNA Isolation Kit
BS8225	Bio Basic	50Preps	EUR 76.64
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

Rapid Yeast Genomic DNA Extraction Kit
BS8227	Bio Basic	50Preps	EUR 76.64
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

Rapid Blood Genomic DNA Extraction Kit
BT4782	Bio Basic	50Preps	EUR 76.64
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

Rapid Fungal Genomic DNA Isolation Kit
FT71415	Bio Basic	50Preps	EUR 76.64
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Fungi

Genomic DNA from Human Tumor Cell Line: A431
D1255801	Biochain	100 ug	EUR 243
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Human Tumor Cell Line: Hela
D1255811	Biochain	100 ug	EUR 243
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Human Tumor Cell Line: Jurkat
D1255815	Biochain	100 ug	EUR 243
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Human Tumor Cell Line: Raji
D1255840	Biochain	100 ug	EUR 243
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Adult Normal Tissue: Colon
D2234090	Biochain	2 ug	EUR 599
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Adult Normal Tissue: Liver
D2234149	Biochain	2 ug	EUR 599
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

FFPE Genomic DNA - Human Adult Normal Tissue: Pancreas
D2234188	Biochain	2 ug	EUR 599
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

VAHTS Human Genomic DNA Quantiﬁcation and QC Kit
NQ201	Vazyme	300 rxn	EUR 575

LumiPure genomic DNA from AnySample Kit, 50
21573	lumiprobe	50 preps	EUR 108

Genomic DNA from Mouse Normal Tissue: Brain
D1334035	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Heart
D1334122	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Kidney
D1334142	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Liver
D1334149	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Spleen
D1334246	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Stomach
D1334248	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Brain
D1434035	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Heart
D1434122	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Kidney
D1434142	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Liver
D1434149	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Stomach
D1434248	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Testis
D1434260	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Thymus
D1434264	Biochain	100 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Arabidopsis
D1634310	Biochain	10 ug	EUR 493
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Arabidopsis
D1634310-5	Biochain	5 ug	EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Corn
D1634330	Biochain	10 ug	EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Orange
D1634340	Biochain	10 ug	EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Potato
D1634350	Biochain	10 ug	EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Rice
D1634360	Biochain	10 ug	EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Plant Normal Tissue: Wheat
D1634390	Biochain	10 ug	EUR 222
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

96 Well Breast Tumor Genomic DNA Plate
D8235086-1	Biochain	1 plate	EUR 316

96 Well Colon Tumor Genomic DNA Plate
D8235090-1	Biochain	1 plate	EUR 316

The outcomes of Western blot urged that the polyclonal antibody is extremely particular to rooster LKB1 protein. Immunofluorescence indicated that the LKB1 protein is principally expressed within the cytoplasm of liver, coronary heart and hypothalamus cells of rooster. Our research confirmed that the LKB1 polyclonal antibodies produced by this methodology are efficient and can be utilized to additional research the function of LKB1 within the pathogenesis of rooster illness.